Automated Guided Vehicle System (AGVS)provides the flexibility and automation demanded byFlexible Manufacturing System (FMS). However, with thegrowing concern on responsible management of resourceuse, it is crucial to manage these vehicles in an efficientway in order reduces travel time and controls conflicts andcongestions. This paper presents the development processof a new Memetic algorithm (MA) for optimizing partitioningproblem of tandem AGVS. MAs employ a Geneticalgorithm (GA), as a global search, and apply a localsearch to bring the solutions to a local optimum point. Anew Tabu Search (TS) has been developed and combinedwith a GA to refine the newly generated individuals by GA. The aim of the proposed algorithm is to minimize themaximum workload of the system. After all, the performanceof the proposed algorithm is evaluated using Matlab. This study also compared the objective function of theproposed MA with GA. The results showed that the TS, asa local search, significantly improves the objective functionof the GA for different system sizes with large and smallnumbers of zone by 1. 26 in average.